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RAGE antibody

Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611
Anti-RAGE antibody used in Western Blot (WB). GTX23611
Anti-RAGE antibody used in Western Blot (WB). GTX23611
Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611
Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611
Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611

Cat No. GTX23611

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat, Frog, Squirrel
Package
100 μl ($319)

APPLICATION

Application Note

Recommended Starting Dilutions: For ICC/IF: Use at a dilution of 1:200. For WB: 1:500-1:10000. For IHC-Fr: Use at a concentration of 1-2 ug/ml. For IHC-P: 1:100-1:1000. Optimal dilution / concentration should be determined by the end user.

Positive Control

HUVEC , mouse lung , rat lung

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_385109

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Advanced Glycosylation End Product-Specific Receptor , Rage , Ager

Research Area

DATA IMAGES

Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611

GTX23611 IHC-P Image

RAGE antibody detects RAGE protein in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
RAGE antibody (GTX23611) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAGE antibody used in Western Blot (WB). GTX23611

GTX23611 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAGE antibody (GTX23611) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAGE antibody used in Western Blot (WB). GTX23611

GTX23611 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RAGE antibody (GTX23611) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611

GTX23611 IHC-P Image

IHC-P analysis of normal mouse kidney tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611

GTX23611 IHC-P Image

IHC-P analysis of normal mouse lymph node tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAGE antibody used in IHC (Paraffin sections) (IHC-P). GTX23611

GTX23611 IHC-P Image

IHC-P analysis of normal mouse heart tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

RAGE antibody Cat No. GTX23611

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016.PMID: 27121132
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Squirrel
Figure Note : The data was peer reviewed and published in the journal PeerJ in 2018.PMID: 29888131
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 26739898
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319