GeneTex
United States (US)

RAGE antibody

GTX23611_IHC_2_18121410_722.jpg
GTX23611_IHC_3_18121410_437.jpg
GTX23611_IHC_1_18121410_732.jpg
GTX23611_41906_20160616_WB_M_lung_18121410_524.jpg
GTX23611_41906_20160616_WB_R_lung_18121410_256.jpg
GTX23611_41906_20160616_WB_18121410_730.jpg
GTX23611_41906_20160601_IHC-P_R_18121410_938.jpg

Cat No. GTX23611

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Squirrel
APPLICATION

Application Note

Recommended Starting Dilutions: For ICC/IF: Use at a dilution of 1:200. For WB: 1:500-1:10000. For IHC-Fr: Use at a concentration of 1-2 ug/ml. For IHC-P: 1:100-1:1000. Optimal dilution / concentration should be determined by the end user.

Positive Control

HUVEC , mouse lung , rat lung
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.

Purification

Antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX23611_IHC_2_18121410_722.jpg

GTX23611 IHC-P Image

IHC-P analysis of normal mouse kidney tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX23611_IHC_3_18121410_437.jpg

GTX23611 IHC-P Image

IHC-P analysis of normal mouse lymph node tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX23611_IHC_1_18121410_732.jpg

GTX23611 IHC-P Image

IHC-P analysis of normal mouse heart tissue with (ledt) or without (right) RAGE antibody at a dilution of 1:20. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX23611_41906_20160616_WB_M_lung_18121410_524.jpg

GTX23611 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAGE antibody (GTX23611) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX23611_41906_20160616_WB_R_lung_18121410_256.jpg

GTX23611 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with RAGE antibody (GTX23611) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX23611_41906_20160616_WB_18121410_730.jpg

GTX23611 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with RAGE antibody (GTX23611) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX23611_41906_20160601_IHC-P_R_18121410_938.jpg

GTX23611 IHC-P Image

RAGE antibody detects RAGE protein in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
RAGE antibody (GTX23611) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf