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RALBP1 antibody [GT1337]

Recombinant antibody
Anti-RALBP1 antibody [GT1337] used in Western Blot (WB). GTX03249
Anti-RALBP1 antibody [GT1337] used in Western Blot (WB). GTX03249
Anti-RALBP1 antibody [GT1337] used in IHC (Paraffin sections) (IHC-P). GTX03249
Anti-RALBP1 antibody [GT1337] used in Western Blot (WB). GTX03249
Anti-RALBP1 antibody [GT1337] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03249
Anti-RALBP1 antibody [GT1337] used in IHC (Paraffin sections) (IHC-P). GTX03249
Anti-RALBP1 antibody [GT1337] used in Western Blot (WB). GTX03249
Anti-RALBP1 antibody [GT1337] used in IHC (Paraffin sections) (IHC-P). GTX03249

Cat. No. GTX03249

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1337

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all RALBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Observed MW

95 kDa. The observed M.W. is based on the following publication. PMID: 16024601

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human RALBP1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ralA binding protein 1 , RIP1 , RLIP1 , RLIP76

Cellular Localization

Membrane

Background

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-RALBP1 antibody [GT1337] used in Western Blot (WB). GTX03249

GTX03249 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RALBP1 antibody [GT1337] (GTX03249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 16024601

Anti-RALBP1 antibody [GT1337] used in Western Blot (WB). GTX03249

GTX03249 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RALBP1 antibody [GT1337] (GTX03249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 16024601

Anti-RALBP1 antibody [GT1337] used in IHC (Paraffin sections) (IHC-P). GTX03249

GTX03249 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX03249 RALBP1 antibody [GT1337].
Dilution : 1:100

Anti-RALBP1 antibody [GT1337] used in Western Blot (WB). GTX03249

GTX03249 WB Image

WB analysis of THP-1 whole cell lysate using GTX03249 RALBP1 antibody [GT1337].
Dilution : 1:1000
Loading : 25μg per lane

Anti-RALBP1 antibody [GT1337] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03249

GTX03249 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03249 RALBP1 antibody [GT1337].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-RALBP1 antibody [GT1337] used in IHC (Paraffin sections) (IHC-P). GTX03249

GTX03249 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX03249 RALBP1 antibody [GT1337].
Dilution : 1:100

Anti-RALBP1 antibody [GT1337] used in Western Blot (WB). GTX03249

GTX03249 WB Image

WB analysis of C6 whole cell lysate using GTX03249 RALBP1 antibody [GT1337].
Dilution : 1:500
Loading : 25μg per lane

Anti-RALBP1 antibody [GT1337] used in IHC (Paraffin sections) (IHC-P). GTX03249

GTX03249 IHC-P Image

IHC-P analysis of human lung cancer section using GTX03249 RALBP1 antibody [GT1337].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439