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RALY antibody

Anti-RALY antibody used in IHC (Paraffin sections) (IHC-P). GTX117389
Anti-RALY antibody used in IHC (Paraffin sections) (IHC-P). GTX117389
Anti-RALY antibody used in Western Blot (WB). GTX117389
Anti-RALY antibody used in IHC (Paraffin sections) (IHC-P). GTX117389
Anti-RALY antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117389

Cat. No. GTX117389

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RALY products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Zebrafish, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RALY. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179429

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RALY heterogeneous nuclear ribonucleoprotein , HNRPCL2 , P542

Cellular Localization

Nucleus

Background

In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RALY antibody used in IHC (Paraffin sections) (IHC-P). GTX117389

GTX117389 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using RALY(GTX117389) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RALY antibody used in IHC (Paraffin sections) (IHC-P). GTX117389

GTX117389 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using RALY(GTX117389) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RALY antibody used in Western Blot (WB). GTX117389

GTX117389 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX117389 diluted at 1:1000

Anti-RALY antibody used in IHC (Paraffin sections) (IHC-P). GTX117389

GTX117389 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RALY(GTX117389) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RALY antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117389

GTX117389 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RALY(GTX117389) antibody at 1:500 dilution.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169