Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAMP1 antibody [C1C3] (GTX108525) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX108525 diluted at 1:1000