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RAMP1 antibody

Anti-RAMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54684
Anti-RAMP1 antibody used in Western Blot (WB). GTX54684
Anti-RAMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54684

Cat. No. GTX54684

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all RAMP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 27-117 of human RAMP1 (NP_005846.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor activity modifying protein 1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES

Anti-RAMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54684

GTX54684 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54684 RAMP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-RAMP1 antibody used in Western Blot (WB). GTX54684

GTX54684 WB Image

WB analysis of HepG2 cell lysate using GTX54684 RAMP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RAMP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54684

GTX54684 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54684 RAMP1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399