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RAN antibody, N-term

Anti-RAN antibody, N-term used in Western Blot (WB). GTX17033
Anti-RAN antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX17033

Cat No. GTX17033

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX17033-PEP).

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence AAQGEPQVQFKLV-C, from the N Terminus of the protein sequence according to NP_006316.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAN, member RAS oncogene family , ARA24 , Gsp1 , TC4

Cellular Localization

Nucleus , Cytoplasm, cytosol , Cytoplasm

Background

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAN antibody, N-term used in Western Blot (WB). GTX17033

GTX17033 WB Image

WB analysis of A431 lysate using GTX17033 RAN antibody, N-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-RAN antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX17033

GTX17033 IHC-P Image

IHC-P analysis of human thymus using GTX17033 RAN antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 369