Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAN(GTX114139) antibody at 1:200 dilution.

RAN antibody detects RAN protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN antibody (GTX114139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX114139 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.