RANBP2 antibody [2E1]

Cat. No. GTX79468

Host	Mouse
Clonality	Monoclonal
Clone Name	2E1
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX79468 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:100 - 1:1000
ICC/IF	1:100-1:1000
IHC-P	1:100 - 1:1000

Not tested in other applications.

Calculated MW

358 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to 150 N-terminal residues of human Nup358.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_11173805

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAN binding protein 2 , ADANE , ANE1 , IIAE3 , NUP358 , TRP1 , TRP2

Cellular Localization

Nucleus

Background

RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5903 RANBP2
UniProt: P49792 RANBP2

Research Area

DATA IMAGES

GTX79468 WB Image

WB analysis of 25 ug of cytoplasmic and nuclear HeLa cell using GTX79468 RANBP2 antibody [2E1].
Dilution : 1:1000

GTX79468 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX79468 RANBP2 antibody [2E1].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS
Dilution : 10 μg/ml in 1% Blocker BSA in PBS

GTX79468 IHC-P Image

IHC-P analysis of human stomach tissue using GTX79468 RANBP2 antibody [2E1].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty