APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
Not tested in other applications.
Calculated MW
Positive Control
MDA-MB-231 membrane extract , DDDDK-tagged TNFRSF11A-transfected 293T
Product Note
Based on internal testing, this antibody is specific for RNAK (TNFRSF11A) and does not cross with OPG (TNFRSF11B).
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.06 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human RANK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887111
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TNF receptor superfamily member 11a , CD265 , FEO , LOH18CR1 , ODFR , OFE , OPTB7 , OSTS , PDB2 , RANK , TRANCER
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Studies of the mouse counterpart suggest that this receptor directly mediates the osteoprotegerin ligand (OPGL)-induced osteoclastogenesis in osteoclast precursor cells. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX133795 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RANK antibody (GTX133795) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133795 WB Image
MDA-MB-231 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RANK antibody (GTX133795) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for RANK antibody (GTX133795). Be the first to share your publications with this product.
REVIEW
RANK antibody
Cat. No. GTX133795
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 30-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
HEK293 cells
Amount used :
15 μg
Blocking :
5% skim milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 18Hr
