GeneTex
United States (US)

RANKL antibody [C2C3], C-term

GTX108515_39812_20150123_IHC_19011507_433.jpg
GTX108515_WB_19011507_993.jpg
GTX108515_39812_WB_R_lung_19011507_295.jpg

Cat No. GTX108515

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Rat lung

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RANKL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD254 antibody, ODF antibody, OPGL antibody, OPTB2 antibody, RANKL antibody, TRANCE antibody, hRANKL2 antibody, sOdf antibody, TNFSF11 antibody, osteoprotegerin ligand antibody, receptor activator of nuclear factor kappa-B ligand antibody, receptor activator of nuclear factor kappa B ligand antibody, tumor necrosis factor ligand superfamily member 11 antibody, osteoclast differentiation factor antibody, TNF-related activation-induced cytokine antibody, "tumor necrosis factor (ligand) superfamily, member 11 antibody"

Cellular Localization

Isoform 1: Cell membrane; Single-pass type II membrane protein , Isoform 3: Cell membrane; Single-pass type II membrane protein , Isoform 2: Cytoplasm , Tumor necrosis factor ligand superfamily member 11 , soluble form: Secreted

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX108515_39812_20150123_IHC_19011507_433.jpg

GTX108515 IHC-P Image

RANKL antibody [C2C3], C-term detects RANKL protein at membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RANKL antibody [C2C3], C-term (GTX108515) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108515_WB_19011507_993.jpg

GTX108515 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
10% SDS PAGE
RANKL (CD254) antibody
GTX108515 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108515_39812_WB_R_lung_19011507_295.jpg

GTX108515 WB Image

RANKL antibody [C2C3], C-term detects RANKL protein by western blot analysis.
A. 50 µg rat lung extract
10% SDS-PAGE
RANKL antibody [C2C3], C-term (GTX108515) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf