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RANKL antibody [C2C3], C-term

Anti-RANKL antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108515
Anti-RANKL antibody [C2C3], C-term used in Western Blot (WB). GTX108515
Anti-RANKL antibody [C2C3], C-term used in Western Blot (WB). GTX108515

Cat No. GTX108515

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Rat lung

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RANKL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952310

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF superfamily member 11 , CD254 , ODF , OPGL , OPTB2 , RANKL , TNLG6B , TRANCE , hRANKL2 , sOdf

Cellular Localization

Isoform 1: Cell membrane; Single-pass type II membrane protein , Isoform 3: Cell membrane; Single-pass type II membrane protein , Isoform 2: Cytoplasm , Tumor necrosis factor ligand superfamily member 11 , soluble form: Secreted

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RANKL antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108515

GTX108515 IHC-P Image

RANKL antibody [C2C3], C-term detects RANKL protein at membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RANKL antibody [C2C3], C-term (GTX108515) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RANKL antibody [C2C3], C-term used in Western Blot (WB). GTX108515

GTX108515 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
RANKL (CD254) antibody
GTX108515 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RANKL antibody [C2C3], C-term used in Western Blot (WB). GTX108515

GTX108515 WB Image

RANKL antibody [C2C3], C-term detects RANKL protein by western blot analysis.
A. 50 μg rat lung extract
10% SDS-PAGE
RANKL antibody [C2C3], C-term (GTX108515) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169