GeneTex

  • Country / Location Selection

United States (US)

RANKL antibody [C2C3], C-term

Anti-RANKL antibody [C2C3], C-term used in Western Blot (WB). GTX108515
Anti-RANKL antibody [C2C3], C-term used in Western Blot (WB). GTX108515
Anti-RANKL antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108515

Cat. No. GTX108515

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RANKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa , rat lung extract

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human RANKL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952310

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF superfamily member 11 , CD254 , ODF , OPGL , OPTB2 , RANKL , TNLG6B , TRANCE , hRANKL2 , sOdf

Cellular Localization

Isoform 1: Cell membrane; Single-pass type II membrane protein , Isoform 3: Cell membrane; Single-pass type II membrane protein , Isoform 2: Cytoplasm , Tumor necrosis factor ligand superfamily member 11 , soluble form: Secreted

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RANKL antibody [C2C3], C-term used in Western Blot (WB). GTX108515

GTX108515 WB Image

RANKL antibody [C2C3], C-term detects RANKL protein by western blot analysis.
A. 50 μg rat lung extract
10% SDS-PAGE
RANKL antibody [C2C3], C-term (GTX108515) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RANKL antibody [C2C3], C-term used in Western Blot (WB). GTX108515

GTX108515 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
RANKL (CD254) antibody
GTX108515 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RANKL antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108515

GTX108515 IHC-P Image

RANKL antibody [C2C3], C-term detects RANKL protein at membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RANKL antibody [C2C3], C-term (GTX108515) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Tenshin H et al. Clin Transl Immunology 2022; 11 (1) : e1371 TGF-β-activated kinase-1 inhibitor LL-Z1640-2 reduces joint inflammation and bone destruction in mouse models of rheumatoid arthritis by inhibiting NLRP3 inflammasome, TACE, TNF-α and RANKL expression.
Reactivity : Mouse
Jiah Yeom et al. Microorganisms 2021; 9 (4) Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio.
Application : WB
Reactivity : Human
Malta FS et al. PLoS One 2020; 15 (8) : e0237660
Application : IHC-P
Reactivity : Rat
Zhang Y et al. Ecotoxicol Environ Saf 2019; 183 : 109441 Impacts of lead exposure and chelation therapy on bone metabolism during different developmental stages of rats.
Application : WB
Reactivity : Rat
Chang MC et al. Int J Mol Sci 2018; 19 (12) Butyrate Stimulates Histone H3 Acetylation, 8-Isoprostane Production, RANKL Expression, and Regulated Osteoprotegerin Expression/Secretion in MG-63 Osteoblastic Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for RANKL antibody [C2C3], C-term (GTX108515). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169