APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Molt-4
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human RANKL. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952313
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TNF superfamily member 11 , CD254 , ODF , OPGL , OPTB2 , RANKL , TNLG6B , TRANCE , hRANKL2 , sOdf
Cellular Localization
Isoform 1: Cell membrane; Single-pass type II membrane protein , Isoform 3: Cell membrane; Single-pass type II membrane protein , Isoform 2: Cytoplasm , Tumor necrosis factor ligand superfamily member 11 , soluble form: Secreted
Background
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-RANKL antibody [N1], N-term used in Western Blot (WB). GTX108516](/templates/default/js/lightbox/images/loading.gif "Anti-RANKL antibody [N1], N-term used in Western Blot (WB). GTX108516")
|
GTX108516 WB Image
Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX108516 diluted at 1:1000
|
REFERENCE
There are currently no references for RANKL antibody [N1], N-term (GTX108516). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RANKL antibody [N1], N-term (GTX108516). Be the first to share your experience with this product.
![Anti-RANKL antibody [N1], N-term used in Western Blot (WB). GTX108516](/upload/website/prouct_img/small/GTX108516/GTX108516_WB_w_23060120_886.webp "Anti-RANKL antibody [N1], N-term used in Western Blot (WB). GTX108516")
![Anti-RANKL antibody [N1], N-term used in Western Blot (WB). GTX108516](/upload/website/prouct_img/normal/GTX108516/GTX108516_WB_w_23060120_886.webp "Anti-RANKL antibody [N1], N-term used in Western Blot (WB). GTX108516")