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RANTES antibody (Biotin)

Anti-RANTES antibody (Biotin) used in ELISA (ELISA). GTX12766
Anti-RANTES antibody (Biotin) used in ELISA (ELISA). GTX12766

Cat. No. GTX12766

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, Sandwich ELISA

Reactivity

 Human
Package
25 μg ($399)
See all RANTES products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 0.2 μg/mL
ELISA 0.15 - 0.30 ng/mL
Sandwich ELISA 0.25 - 1.0 ug/ml

Note :

Sandwich ELISA
Capture : Anti-Human RANTES antibody, Detection : GTX12766
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hRANTES (human RANTES).

Purification

Purified by affinity chromatography

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-C motif chemokine ligand 5 , D17S136E , RANTES , SCYA5 , SIS-delta , SISd , TCP228 , eoCP

Cellular Localization

Secreted

Background

RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV-suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). It is T-cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

Database

Research Area

DATA IMAGES

Anti-RANTES antibody (Biotin) used in ELISA (ELISA). GTX12766

GTX12766 ELISA Image

ELISA analysis of human RANTES recombinant protein(0.2 - 0.4 ng/well) using GTX12766 RANTES antibody (Biotin)(detection antibody) at 0.25- 1.0 ug/ml and Anti-Human RANTES as a capture antibody.

Anti-RANTES antibody (Biotin) used in ELISA (ELISA). GTX12766

GTX12766 ELISA Image

ELISA analysis of human RANTES recombinant proteint (0.2 – 0.4 ng/well) using GTX12766 RANTES antibody (Biotin).
Working concentration : 0.25- 1.0 ug/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399