GeneTex
United States (US)

RAP1 antibody [N2C3]

GTX101983_40163_IHC_19011506_277.jpg
GTX101983_WB_19011506_811.jpg
GTX101983_40163_IP_19011506_261.jpg
GTX101983_40898_IFA_19011506_846.jpg
GTX101983_40163_WB_M_brain_19011506_382.jpg

Cat No. GTX101983

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , mouse brain

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Terf2 Interacting Protein,Drip5,Rap1,Terf2Ip

Cellular Localization

Nucleus , Telomere

Background

The gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22. [provided by RefSeq]

Database

DATA IMAGES
GTX101983_40163_IHC_19011506_277.jpg

GTX101983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RAP1(GTX101983) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101983_WB_19011506_811.jpg

GTX101983 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: HCT116
10% SDS PAGE
RAP1 antibody
GTX101983 diluted at 1:1000

GTX101983_40163_IP_19011506_261.jpg

GTX101983 IP Image

RAP1 antibody immunoprecipitates RAP1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of RAP1 by 3 μg of RAP1 antibody (GTX101983) 10% SDS-PAGE The immunoprecipitated RAP1 protein was detected by RAP1 antibody (GTX101983) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX101983_40898_IFA_19011506_846.jpg

GTX101983 ICC/IF Image

RAP1 antibody [N2C3] detects RAP1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAP1 protein stained by RAP1 antibody [N2C3] (GTX101983) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101983_40163_WB_M_brain_19011506_382.jpg

GTX101983 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101983 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169