RAP1 antibody immunoprecipitates RAP1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of RAP1 by 3 μg of RAP1 antibody (GTX101983) 10% SDS-PAGE The immunoprecipitated RAP1 protein was detected by RAP1 antibody (GTX101983) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101983 diluted at 1:1000

RAP1 antibody [N2C3] detects RAP1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAP1 protein stained by RAP1 antibody [N2C3] (GTX101983) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RAP1(GTX101983) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP1 antibody [N2C3] (GTX101983) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.