Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAP1 antibody (GTX102770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1 antibody (GTX102770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.