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RAP1A antibody [5F8G2]

Anti-RAP1A antibody [5F8G2] used in Flow cytometry (FACS). GTX60586
Anti-RAP1A antibody [5F8G2] used in Western Blot (WB). GTX60586
Anti-RAP1A antibody [5F8G2] used in Western Blot (WB). GTX60586
Anti-RAP1A antibody [5F8G2] used in ELISA (ELISA). GTX60586

Cat. No. GTX60586

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F8G2

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all RAP1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human RAP1A expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAP1A, member of RAS oncogene family , C21KG , G-22K , KREV-1 , KREV1 , RAP1 , SMGP21

Cellular Localization

Cell membrane,Cytoplasm,Cell junction

Background

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAP1A antibody [5F8G2] used in Flow cytometry (FACS). GTX60586

GTX60586 FACS Image

FACS analysis of HeLa cells using GTX60586 RAP1A antibody [5F8G2].
Green : RAP1A
Red : negative control

Anti-RAP1A antibody [5F8G2] used in Western Blot (WB). GTX60586

GTX60586 WB Image

WB analysis of HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate using GTX60586 RAP1A antibody [5F8G2].

Anti-RAP1A antibody [5F8G2] used in Western Blot (WB). GTX60586

GTX60586 WB Image

WB analysis of human RAP1A (AA: 28-180) recombinant protein using GTX60586 RAP1A antibody [5F8G2].

Anti-RAP1A antibody [5F8G2] used in ELISA (ELISA). GTX60586

GTX60586 ELISA Image

ELISA analysis of antigen using GTX60586 RAP1A antibody [5F8G2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409