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RAP1B antibody

Overexpression
Anti-RAP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX101950
Anti-RAP1B antibody used in Western Blot (WB). GTX101950
Anti-RAP1B antibody used in Western Blot (WB). GTX101950

Cat. No. GTX101950

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAP1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HCT116 , RAP1B-transfected 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAP1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630490

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAP1B, member of RAS oncogene family , K-REV , RAL1B

Background

RAP1B and RAP1A (MIM 179520) belong to a superfamily of RAS (see MIM 190020)-like small GTP-binding proteins involved in cell signaling.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RAP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX101950

GTX101950 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using RAP1B(GTX101950) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RAP1B antibody used in Western Blot (WB). GTX101950

GTX101950 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX101950 diluted at 1:500

Anti-RAP1B antibody used in Western Blot (WB). GTX101950

GTX101950 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1B antibody (GTX101950) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169