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RAP1B antibody

Anti-RAP1B antibody used in Western Blot (WB). GTX66139
Anti-RAP1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66139

Cat. No. GTX66139

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all RAP1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-184 of human RAP1B (NP_056461.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAP1B, member of RAS oncogene family , K-REV , RAL1B

Cellular Localization

Cell membrane,Cytoplasm, cytosol,Cell junction

Background

This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-RAP1B antibody used in Western Blot (WB). GTX66139

GTX66139 WB Image

WB analysis of various sample lysates using GTX66139 RAP1B antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:3000
Loading : 25μg per lane

Anti-RAP1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66139

GTX66139 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX66139 RAP1B antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399