GeneTex

  • Country / Location Selection

United States (US)

RAP2A antibody [N1C3-2]

Anti-RAP2A antibody [N1C3-2] used in Western Blot (WB). GTX108831
Anti-RAP2A antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108831
Anti-RAP2A antibody [N1C3-2] used in Western Blot (WB). GTX108831
Anti-RAP2A antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108831

Cat. No. GTX108831

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAP2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Sheep, Bovine, Xenopus laevis, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RAP2A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726416

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAP2A, member of RAS oncogene family , K-REV , KREV , RAP2 , RbBP-30

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.

Database

Research Area

DATA IMAGES

Anti-RAP2A antibody [N1C3-2] used in Western Blot (WB). GTX108831

GTX108831 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAP2A antibody [N1C3-2] (GTX108831) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAP2A antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108831

GTX108831 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using RAP2A(GTX108831) antibody at 1:500 dilution.

Anti-RAP2A antibody [N1C3-2] used in Western Blot (WB). GTX108831

GTX108831 WB Image

The data was published in the journal PLoS One in 2013. PMID: 23894443

Anti-RAP2A antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108831

GTX108831 ICC/IF Image

The data was published in the journal PLoS One in 2013. PMID: 23894443

REFERENCE

Application Reference

Takino JI et al. Sci Rep 2019; 9 (1) : 16717 The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells.
Application : WB
Reactivity : Human
Telkoparan P et al. PLoS One 2013; 8 (7) : e69289 Coiled-coil domain containing protein 124 is a novel centrosome and midbody protein that interacts with the Ras-guanine nucleotide exchange factor 1B and is involved in cytokinesis.
Submit a Reference

REVIEW

There are currently no reviews for RAP2A antibody [N1C3-2] (GTX108831). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169