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RAP2A antibody [N1C3-2]

Anti-RAP2A antibody [N1C3-2] used in Western Blot (WB). GTX108831
Anti-RAP2A antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108831

Cat No. GTX108831

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , MCF-7

Predict Reactivity

Mouse, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human RAP2A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726416

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAP2A, member of RAS oncogene family , K-REV , KREV , RAP2 , RbBP-30

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.

Database

Research Area

DATA IMAGES

Anti-RAP2A antibody [N1C3-2] used in Western Blot (WB). GTX108831

GTX108831 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAP2A antibody [N1C3-2] (GTX108831) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAP2A antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108831

GTX108831 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using RAP2A(GTX108831) antibody at 1:500 dilution.

REFERENCE

REVIEW

RAP2A antibody [N1C3-2] Cat No. GTX108831

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 23894443
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 23894443
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169