GeneTex
United States (US)

RAP2B antibody

GTX114702_40219_IHC_18121410_240.jpg
GTX114702_WB_18121410_558.jpg
GTX114702_40219_WB_M_brain_18121410_972.jpg

Cat No. GTX114702

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A431 , H1299 , HepG2 , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAP2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC20484 antibody, RAP2B antibody, small GTP binding protein antibody, Ras family small GTP binding protein RAP2B antibody, ras-related protein Rap-2b antibody, "RAP2B, member of RAS oncogene family antibody"

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq]
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Database

DATA IMAGES
GTX114702_40219_IHC_18121410_240.jpg

GTX114702 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using RAP2B(GTX114702) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114702_WB_18121410_558.jpg

GTX114702 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX114702 diluted at 1:1000

GTX114702_40219_WB_M_brain_18121410_972.jpg

GTX114702 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114702 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169