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RAS (A59T Mutant) antibody

Anti-RAS (A59T Mutant) antibody used in Western Blot (WB). GTX132403

Cat No. GTX132403

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Human Ras (A59T Mutant specific)-transfected 293T whole cell extracts

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Guinea pig, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Ras (mutant A59T). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
Anti-RAS (A59T Mutant) antibody used in Western Blot (WB). GTX132403

GTX132403 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (A59T Mutant Specific) antibody (GTX132403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169