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RAS (G12A mutant) antibody [HL1153]

Recombinant antibody Overexpression
Anti-RAS (G12A mutant) antibody [HL1153] used in Western Blot (WB). GTX636434
Anti-RAS (G12A mutant) antibody [HL1153] used in Western Blot (WB). GTX636434

Cat. No. GTX636434

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1153

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RAS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Predict Reactivity

Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant G12A of human K-Ras. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909982

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HRas proto-oncogene, GTPase , KRAS proto-oncogene, GTPase , NRAS proto-oncogene, GTPase , C-BAS/HAS , C-H-RAS , C-HA-RAS1 , CTLO , H-RASIDX , HAMSV , HRAS1 , KRAS , KRAS2 , Ki-Ras , RASH1 , RASK2 , c-K-ras , c-Ki-ras , p21ras , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2 , ALPS4 , CMNS , N-ras , NCMS , NRAS1 , NS6

Cellular Localization

Golgi apparatus,Cytoplasm,Cell membrane,Golgi apparatus membrane,Cytoplasm, cytosol

Database

Research Area

DATA IMAGES

Anti-RAS (G12A mutant) antibody [HL1153] used in Western Blot (WB). GTX636434

GTX636434 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12A mutant) antibody [HL1153] (GTX636434) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12A mutant) antibody [HL1153] used in Western Blot (WB). GTX636434

GTX636434 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12A mutant) antibody [HL1153] (GTX636434) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations