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RAS (G12D Mutant) antibody [HL10]

Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

Cat No. GTX635362

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL10

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($369),
25 μl ($169)
PRODUCT

Summary

KRAS has the distinction of being a preeminent oncoprotein as it is mutated in more than 85% of RAS-altered cancers. The RAS (G12D Mutant) antibody [HL10] is the first commercially available recombinant antibody that demonstrates exceptional specificity by paraffin-embedded immunohistochemistry and western blot for the RAS G12D mutant protein.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:25-1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.54 mg/ml

Antigen Species

Human

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362

GTX635362 IHC-P Image

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human wild-type (left) and KRAS G12D mutant (right) pancreatic tumor.
RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:50.

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Various whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000.

Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362

GTX635362 IHC-P Image

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein at by immunohistochemical analysis.
Sample: Paraffin-embedded KPC mouse pancreatic tumor cells implanted in the pancreas of B6 mice (left) and normal mouse pancreas (right).
RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:75.
Antigen Retrieval: Citrate buffer, pH 6.0, 40 min

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Non-transfected (–) and transfected (+) Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

RAS (G12D Mutant) antibody [HL10] Cat No. GTX635362

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Mar-2020
Score :
Application Tested : ICC/IF
Sample Species : Hu
Fixation : 4% paraformaldehyde, 25°C, 0.25Hr
Primary Antibody : 1:100, 4°C, 16Hr
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : IHC-P
Sample Species : Ms
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.6Hr
Primary Antibody : 1:1:75, 25°C, 0.5Hr
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1500, 25°C, 2Hr
Lane Description : Lane1: PANC1 (KRAS G12D)
Land2: AsPC1 (KRAS G12D)
Land3: BxPC3 (KRAS WT)
Lane4: MIA PaCa-2 (KRAS G12C)
Lane5: ‎CaPan-1 (KRAS G12V)
SDS
PBS.pdf