RAS (G12D Mutant) antibody [HL10]

Cat. No. GTX635362
5 References 7
Cat. No.  GTX635362
Recombinant antibody Orthogonal Overexpression
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
GTX635362 IHC-P Image
Orthogonal

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human wild-type (left) and KRAS G12D mutant (right) pancreatic tumor.
RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:50.

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Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
GTX635362 WB Image
Orthogonal

Various whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000.

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Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
GTX635362 WB Image
Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
GTX635362 IHC-P Image
Orthogonal

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein at by immunohistochemical analysis.
Sample: Paraffin-embedded KPC mouse pancreatic tumor cells implanted in the pancreas of B6 mice (left) and normal mouse pancreas (right).
RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:75.
Antigen Retrieval: Citrate buffer, pH 6.0, 40 min

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Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
GTX635362 WB Image
Overexpression

Non-transfected (–) and transfected (+) Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-RAS (G12D Mutant) antibody [HL10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635362
GTX635362 ICC/IF Image
Overexpression

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635362
  • Host
    Rabbit
  • Clonality
    Monoclonal
  • Clone Name
    HL10
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P
  • Reactivity
    Human, Mouse
Summary
KRAS has the distinction of being a preeminent oncoprotein as it is mutated in more than 85% of RAS-altered cancers. The RAS (G12D Mutant) antibody [HL10] is the first commercially available recombinant antibody that demonstrates exceptional specificity by paraffin-embedded immunohistochemistry and western blot for the RAS G12D mutant protein.
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