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RAS (G12D Mutant) antibody [HL10]

Recombinant antibody Orthogonal Overexpression
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635362

Cat. No. GTX635362

Reference 1
5

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL10

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAS products
PRODUCT

Summary

KRAS has the distinction of being a preeminent oncoprotein as it is mutated in more than 85% of RAS-altered cancers. The RAS (G12D Mutant) antibody [HL10] is the first commercially available recombinant antibody that demonstrates exceptional specificity by paraffin-embedded immunohistochemistry and western blot for the RAS G12D mutant protein.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:25-1:100

Note :

WB
Suggest using high sensitivity ECL for optimization. GTX14698 Trident femto Western HRP Substrate is recommended.
Not tested in other applications.

Positive Control

293T transfected NRAS(G12D) , AsPC1 , HPAC , HPAF-II , PANC1 , SU86.86

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant G12D of human K-Ras. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888515

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362

GTX635362 IHC-P Image

Orthogonal

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human wild-type (left) and KRAS G12D mutant (right) pancreatic tumor.
RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:50.

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Various whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000.

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Overexpression

Non-transfected (–) and transfected (+) Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362

GTX635362 IHC-P Image

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein at by immunohistochemical analysis.
Sample: Paraffin-embedded KPC mouse pancreatic tumor cells implanted in the pancreas of B6 mice (left) and normal mouse pancreas (right).
RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:75.
Antigen Retrieval: Citrate buffer, pH 6.0, 40 min

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12D Mutant) antibody [HL10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635362

GTX635362 ICC/IF Image

Orthogonal

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Scott Ferguson et al. Sci Adv 2022; 8 (16) : eabm3453 Single-EV analysis (sEVA) of mutated proteins allows detection of stage 1 pancreatic cancer.
Submit a Reference

REVIEW

RAS (G12D Mutant) antibody [HL10] Cat. No. GTX635362

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 3 users reviews)
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Date : Anonymous submitted on 01-Aug-2022
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : WT and Kras mutant mouse pancreatic tissue sections
Blocking : 5% milk, RT, 1Hr
Fixation : 4% PFA, 48Hr
Permeabilization /Antigen retrieval : Citrate buffer (pH6.0), 20 min, 96°C
Primary Antibody : 1:100 in 1% milk, 4°C, > 12Hr
Figure Note :
The image is from a collaborating laboratory.
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Date : Anonymous submitted on 27-Jan-2022
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Human PDAC lesion (WT and KRAS G12D)
Blocking : 5% milk
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Citrate buffer (pH 6); Autoclaved
Primary Antibody : 1:100 diluted in 1% milk, °C, Hr
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Date : Anonymous submitted on 16-Jun-2020
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 40 μg
Blocking : non-fat dry milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : IHC-P
Sample Species : Ms
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.6Hr
Primary Antibody : 1:1:75, 25°C, 0.5Hr
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1500, 25°C, 2Hr
Lane Description : Lane1: PANC1 (KRAS G12D)
Land2: AsPC1 (KRAS G12D)
Land3: BxPC3 (KRAS WT)
Lane4: MIA PaCa-2 (KRAS G12C)
Lane5: ‎CaPan-1 (KRAS G12V)
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations