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RAS (G12D Mutant) antibody [HL10]

Recombinant antibody Orthogonal Overexpression
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362

Cat. No. GTX635362

Reference 3
5

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL10

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RAS products
PRODUCT

Summary

KRAS has the distinction of being a preeminent oncoprotein as it is mutated in more than 85% of RAS-altered cancers. The RAS (G12D Mutant) antibody [HL10] is the first commercially available recombinant antibody that demonstrates exceptional specificity by paraffin-embedded immunohistochemistry and western blot for the RAS G12D mutant protein.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:25-1:100

Note :

WB
Suggest using high sensitivity ECL for optimization. GTX14698 Trident femto Western HRP Substrate is recommended.
Not tested in other applications.

Positive Control

293T transfected NRAS(G12D) , AsPC1 , HPAC , HPAF-II , PANC1 , SU86.86

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant G12D of human K-Ras. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888515

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362

GTX635362 IHC-P Image

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human wild-type (left) and KRAS G12D mutant (right) pancreatic tumor.
RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:50.

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Various whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000.

Anti-RAS (G12D Mutant) antibody [HL10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635362

GTX635362 ICC/IF Image

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362

GTX635362 WB Image

Overexpression

Non-transfected (–) and transfected (+) Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362

GTX635362 IHC-P Image

RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein at by immunohistochemical analysis.
Sample: Paraffin-embedded KPC mouse pancreatic tumor cells implanted in the pancreas of B6 mice (left) and normal mouse pancreas (right).
RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:75.
Antigen Retrieval: Citrate buffer, pH 6.0, 40 min

REFERENCE

Application Reference

Santofimia-Casta?o P et al. EMBO Mol Med 2024; 16 (3) : 475-505 Targeting NUPR1-dependent stress granules formation to induce synthetic lethality in Kras(G12D)-driven tumors.
Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3) : e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
Scott Ferguson et al. Sci Adv 2022; 8 (16) : eabm3453 Single-EV analysis (sEVA) of mutated proteins allows detection of stage 1 pancreatic cancer.
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REVIEW

RAS (G12D Mutant) antibody [HL10] Cat. No. GTX635362

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 3 users reviews)
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Date : Anonymous submitted on 01-Aug-2022
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : WT and Kras mutant mouse pancreatic tissue sections
Blocking : 5% milk, RT, 1Hr
Fixation : 4% PFA, 48Hr
Permeabilization /Antigen retrieval : Citrate buffer (pH6.0), 20 min, 96°C
Primary Antibody : 1:100 in 1% milk, 4°C, > 12Hr
Figure Note :
The image is from a collaborating laboratory.
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Date : Anonymous submitted on 27-Jan-2022
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Human PDAC lesion (WT and KRAS G12D)
Blocking : 5% milk
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Citrate buffer (pH 6); Autoclaved
Primary Antibody : 1:100 diluted in 1% milk, °C, Hr
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Date : Anonymous submitted on 16-Jun-2020
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 40 μg
Blocking : non-fat dry milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-RAS (G12D Mutant) antibody [HL10] used in IHC (Paraffin sections) (IHC-P). GTX635362
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : IHC-P
Sample Species : Ms
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.6Hr
Primary Antibody : 1:1:75, 25°C, 0.5Hr
Anti-RAS (G12D Mutant) antibody [HL10] used in Western Blot (WB). GTX635362
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1500, 25°C, 2Hr
Lane Description : Lane1: PANC1 (KRAS G12D)
Land2: AsPC1 (KRAS G12D)
Land3: BxPC3 (KRAS WT)
Lane4: MIA PaCa-2 (KRAS G12C)
Lane5: ‎CaPan-1 (KRAS G12V)
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations