APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide of human RAS (G12D Mutant)
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
KRAS proto-oncogene, GTPase , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2
Cellular Localization
Cell membrane,Cytoplasm, cytosol
Background
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-RAS (G12D Mutant) antibody [HL2640] used in Western Blot (WB). GTX639096](/templates/default/js/lightbox/images/loading.gif "Anti-RAS (G12D Mutant) antibody [HL2640] used in Western Blot (WB). GTX639096")
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GTX639096 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639096 ICC/IF Image
RAS (G12D Mutant) antibody [HL2640] detects RAS (G12D Mutant) protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX639096 ICC/IF Image
RAS (G12D Mutant) antibody [HL2640] detects RAS (G12D Mutant) protein at cytoplasm by immunofluorescent analysis.Sample: Aspc-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for RAS (G12D Mutant) antibody [HL2640] (GTX639096). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RAS (G12D Mutant) antibody [HL2640] (GTX639096). Be the first to share your experience with this product.
![Anti-RAS (G12D Mutant) antibody [HL2640] used in Western Blot (WB). GTX639096](/upload/website/prouct_img/small/GTX639096/GTX639096_45257_20231215_WB_23121922_250.webp "Anti-RAS (G12D Mutant) antibody [HL2640] used in Western Blot (WB). GTX639096")
![Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096](/upload/website/prouct_img/small/GTX639096/GTX639096_T-45187_20231124_ICC_IF_B_23121922_372.webp "Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096")
![Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096](/upload/website/prouct_img/small/GTX639096/GTX639096_45257_20240307_ICC_IF_24041019_919.webp "Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096")
![Anti-RAS (G12D Mutant) antibody [HL2640] used in Western Blot (WB). GTX639096](/upload/website/prouct_img/normal/GTX639096/GTX639096_45257_20231215_WB_23121922_250.webp "Anti-RAS (G12D Mutant) antibody [HL2640] used in Western Blot (WB). GTX639096")
![Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096](/upload/website/prouct_img/normal/GTX639096/GTX639096_T-45187_20231124_ICC_IF_B_23121922_372.webp "Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096")
![Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096](/upload/website/prouct_img/normal/GTX639096/GTX639096_45257_20240307_ICC_IF_24041019_919.webp "Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096")