APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:50-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding mutant G12V of human K-Ras. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888545
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
KRAS proto-oncogene, GTPase , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2
Cellular Localization
Cell membrane,Cytoplasm, cytosol
Background
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623](/templates/default/js/lightbox/images/loading.gif "Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623")
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GTX635623 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635623 IHC-P Image
RAS (G12V Mutant) antibody [HL169] detects RAS (G12V Mutant) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BxPC-3 xenograft (left) and CFPAC-1 xenograft (right).
RAS (G12V Mutant) stained by RAS (G12V Mutant) antibody [HL169] (GTX635623) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635623 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12V Mutant) antibody [HL169] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635623 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for RAS (G12V Mutant) antibody [HL169] (GTX635623). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RAS (G12V Mutant) antibody [HL169] (GTX635623). Be the first to share your experience with this product.
![Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623](/upload/website/prouct_img/small/GTX635623/GTX635623_44060_20210924_WB_21100100_103.jpg "Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623")
![Anti-RAS (G12V Mutant) antibody [HL169] used in IHC (Paraffin sections) (IHC-P). GTX635623](/upload/website/prouct_img/small/GTX635623/GTX635623_44347_20220617_IHC-P_22062121_883.webp "Anti-RAS (G12V Mutant) antibody [HL169] used in IHC (Paraffin sections) (IHC-P). GTX635623")
![Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623](/upload/website/prouct_img/small/GTX635623/GTX635623_44879_20221209_WB_23032819_246.webp "Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623")
![Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623](/upload/website/prouct_img/small/GTX635623/GTX635623_44060_20210319_WB_B_21032603_150.jpg "Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623")
![Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623](/upload/website/prouct_img/normal/GTX635623/GTX635623_44060_20210924_WB_21100100_103.jpg "Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623")
![Anti-RAS (G12V Mutant) antibody [HL169] used in IHC (Paraffin sections) (IHC-P). GTX635623](/upload/website/prouct_img/normal/GTX635623/GTX635623_44347_20220617_IHC-P_22062121_883.webp "Anti-RAS (G12V Mutant) antibody [HL169] used in IHC (Paraffin sections) (IHC-P). GTX635623")
![Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623](/upload/website/prouct_img/normal/GTX635623/GTX635623_44879_20221209_WB_23032819_246.webp "Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623")
![Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623](/upload/website/prouct_img/normal/GTX635623/GTX635623_44060_20210319_WB_B_21032603_150.jpg "Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623")