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RAS (G12V Mutant) antibody [HL169]

Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623
Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623
Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623
Anti-RAS (G12V Mutant) antibody [HL169] used in IHC (Paraffin sections) (IHC-P). GTX635623

Cat No. GTX635623

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL169

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:50-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant G12V of human K-Ras. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888545

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

KRAS proto-oncogene, GTPase , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2

Cellular Localization

Cell membrane,Cytoplasm, cytosol

Background

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623

GTX635623 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623

GTX635623 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12V Mutant) antibody [HL169] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12V Mutant) antibody [HL169] used in Western Blot (WB). GTX635623

GTX635623 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (GTX635623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12V Mutant) antibody [HL169] used in IHC (Paraffin sections) (IHC-P). GTX635623

GTX635623 IHC-P Image

RAS (G12V Mutant) antibody [HL169] detects RAS (G12V Mutant) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BxPC-3 xenograft (left) and CFPAC-1 xenograft (right).
RAS (G12V Mutant) stained by RAS (G12V Mutant) antibody [HL169] (GTX635623) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for RAS (G12V Mutant) antibody [HL169] (GTX635623). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations