Application Note
IF: Use at a concentration of 2.5 μg/ml. IHC-P: Use at a concentration of 5 μg/ml. Perform enzymatic antigen retrieval (0.05% saponin in water, 30 min. or 0.1% pepsin in 0.1 M HCl, 10-20 min. at room temperature) before commencing with IHC staining protocol. IHC-Fr: Use at a concentration of 5 μg/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2.5 μg/ml. Predicted molecular weight: 21 kDa. Optimal dilutions/concentrations should be determined by the end user.
Product Note
Monoclonal Anti-vH RAS reacts with phosphorylated and non-phosphorylated forms of vH RAS and vK RAS p21's and reacts with the p21 transitional products of the H, K, and N ras human genes. It does not crossreact with Rap. This antibody neutralizes the activity of H, K, and NRas by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70 and Arg-73.
Form
Liquid
Buffer
0.05M Sodium Phosphate pH7.5, 0.2% Gelatin
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full length p21 protein (Human).
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_373286
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
K-RAS Antibody , RASH1 Antibody , HAMSV Antibody , HRAS Antibody , C-HA-RAS1 Antibody , C-BAS/HAS Antibody , N-RAS Antibody , CTLO Antibody , C-H-RAS Antibody , H-RASIDX Antibody , HRAS1 Antibody , C-K-RAS Antibody , NS Antibody , KRAS1 Antibody , K-RAS4A Antibody , KRAS Antibody , KI-RAS Antibody , KRAS2 Antibody , K-RAS2B Antibody , NS3 Antibody , K-RAS2A Antibody , K-RAS4B Antibody , RASK2 Antibody , NRAS Antibody , NRAS1 Antibody , N-RAS Antibody , ALPS4 Antibody , NS6 Antibody
Cellular Localization
Cytoplasmic
Background
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for
Research Area
