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RAS antibody

Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480
Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480

Cat No. GTX132480

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Zebrafish, Goat, Frog, Squirrel
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

A549 , H1299 , HCT116 , KRAS , HRAS , NRAS , zebrafish liver , zebrafish GI , whole zebrafish ,

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KRAS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886657

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480

GTX132480 IHC-P Image

Ras antibody detects Ras protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480

GTX132480 IHC-P Image

Ras antibody detects Ras protein at cell membrane in rat urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded rat urinary bladder.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

RAS antibody Cat No. GTX132480

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : colon cancer tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 24Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169