APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 10 μg/ml |
| 5 μg/ml |
| 1-5 μg |
Not tested in other applications.
Calculated MW
Product Note
Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3.
PROPERTIES
Form
Liquid
Buffer
buffer
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length recombinant human GAP.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RAS p21 protein activator 1 , CM-AVM , CMAVM , CMAVM1 , GAP , PKWS , RASA , RASGAP , p120 , p120GAP , p120RASGAP
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]
Database
Research Area
DATA IMAGES
![Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922](/templates/default/js/lightbox/images/loading.gif "Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922")
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GTX22922 ICC/IF Image
ICC/IF analysis of Hela cells using GTX22922 RASA1 antibody [B4F8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
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GTX22922 ICC/IF Image
ICC/IF analysis of A431 cells using GTX22922 RASA1 antibody [B4F8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
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GTX22922 WB Image
WB analysis of 25 ug of Hela (Lane 1), A431 (Lane 2), and mouse brain cell lysates (Lane 3) using GTX22922 RASA1 antibody [B4F8].
Dilution : 1:200
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GTX22922 ICC/IF Image
ICC/IF analysis of C2C12 cells using GTX22922 RASA1 antibody [B4F8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
|
REFERENCE
There are currently no references for RASA1 antibody [B4F8] (GTX22922). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RASA1 antibody [B4F8] (GTX22922). Be the first to share your experience with this product.
![Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922](/upload/website/prouct_img/small/GTX22922/GTX22922_546_ICC-IF_w_23060620_382.webp "Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922")
![Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922](/upload/website/prouct_img/small/GTX22922/GTX22922_544_ICC-IF_w_23060620_596.webp "Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922")
![Anti-RASA1 antibody [B4F8] used in Western Blot (WB). GTX22922](/upload/website/prouct_img/small/GTX22922/GTX22922_1632_WB_w_23060620_249.webp "Anti-RASA1 antibody [B4F8] used in Western Blot (WB). GTX22922")
![Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922](/upload/website/prouct_img/small/GTX22922/GTX22922_545_ICC-IF_w_23060620_223.webp "Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922")
![Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922](/upload/website/prouct_img/normal/GTX22922/GTX22922_546_ICC-IF_w_23060620_382.webp "Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922")
![Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922](/upload/website/prouct_img/normal/GTX22922/GTX22922_544_ICC-IF_w_23060620_596.webp "Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922")
![Anti-RASA1 antibody [B4F8] used in Western Blot (WB). GTX22922](/upload/website/prouct_img/normal/GTX22922/GTX22922_1632_WB_w_23060620_249.webp "Anti-RASA1 antibody [B4F8] used in Western Blot (WB). GTX22922")
![Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922](/upload/website/prouct_img/normal/GTX22922/GTX22922_545_ICC-IF_w_23060620_223.webp "Anti-RASA1 antibody [B4F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22922")