GeneTex

  • Country / Location Selection

United States (US)

RASA4 antibody

Anti-RASA4 antibody used in Western Blot (WB). GTX121563
Anti-RASA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121563

Cat. No. GTX121563

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RASA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RASA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167044

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAS p21 protein activator 4 , CAPRI , GAPL

Cellular Localization

Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein

Background

This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RASA4 antibody used in Western Blot (WB). GTX121563

GTX121563 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX121563 diluted at 1:3000

Anti-RASA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121563

GTX121563 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using RASA4(GTX121563) antibody at 1:500 dilution.

REFERENCE

There are currently no references for RASA4 antibody (GTX121563). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for RASA4 antibody (GTX121563). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169