APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RASAL1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951642
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
RAS protein activator like 1 , RASAL
Background
The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-RASAL1 antibody [C1C3] used in Western Blot (WB). GTX109520](/templates/default/js/lightbox/images/loading.gif "Anti-RASAL1 antibody [C1C3] used in Western Blot (WB). GTX109520")
|
GTX109520 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX109520 diluted at 1:1000
|
|
|
GTX109520 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using RASAL1(GTX109520) antibody at 1:200 dilution.
|
REFERENCE
There are currently no references for RASAL1 antibody [C1C3] (GTX109520). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RASAL1 antibody [C1C3] (GTX109520). Be the first to share your experience with this product.
![Anti-RASAL1 antibody [C1C3] used in Western Blot (WB). GTX109520](/upload/website/prouct_img/small/GTX109520/GTX109520_WB_19011507_731.jpg "Anti-RASAL1 antibody [C1C3] used in Western Blot (WB). GTX109520")
![Anti-RASAL1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109520](/upload/website/prouct_img/small/GTX109520/GTX109520_IFA_19011507_178.jpg "Anti-RASAL1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109520")
![Anti-RASAL1 antibody [C1C3] used in Western Blot (WB). GTX109520](/upload/website/prouct_img/normal/GTX109520/GTX109520_WB_19011507_731.jpg "Anti-RASAL1 antibody [C1C3] used in Western Blot (WB). GTX109520")
![Anti-RASAL1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109520](/upload/website/prouct_img/normal/GTX109520/GTX109520_IFA_19011507_178.jpg "Anti-RASAL1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109520")