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RASGRF1 antibody

Comparison
Anti-RASGRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133775
Anti-RASGRF1 antibody used in Western Blot (WB). GTX133775
Anti-RASGRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133775

Cat. No. GTX133775

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RASGRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Rat RASGRF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887104

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAS protein-specific guanine nucleotide-releasing factor 1 , GNRP , P140 RAS-GRF

Background

a paternally expressed imprinted gene [RGD]

Database

Research Area

DATA IMAGES

Anti-RASGRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133775

GTX133775 IHC-P Image

RASGRF1 antibody detects RASGRF1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RASGRF1 stained by RASGRF1 antibody (GTX133775) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RASGRF1 antibody used in Western Blot (WB). GTX133775

GTX133775 WB Image

Comparison

Various tissue extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with RASGRF1 antibody (GTX133775) diluted at 1:1000 and competitor's antibody (sc-224) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-RASGRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133775

GTX133775 IHC-P Image

RASGRF1 antibody detects RASGRF1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RASGRF1 stained by RASGRF1 antibody (GTX133775) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169