GeneTex
United States (US)

RASGRF1 antibody

GTX64744_20180530_IHC-P_18121411_523.jpg
GTX64744_20180530_WB_18121411_336.jpg
GTX64744_20180530_ICCIF_18121411_353.jpg

Cat No. GTX64744

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

145 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-190 of human RASGRF1 (NP_001139120.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GNRP Antibody , H GRF55 Antibody , GRF1 Antibody , CDC25 Antibody , GRF55 Antibody , RAS GRF1 Antibody , CDC25L Antibody , PP13187 Antibody

Background

The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES
GTX64744_20180530_IHC-P_18121411_523.jpg

GTX64744 IHC-P Image

IHC-P analysis of human stomach tissue using GTX64744 RASGRF1 antibody.
Dilution : 1:100

GTX64744_20180530_WB_18121411_336.jpg

GTX64744 WB Image

WB analysis of mouse brain tissue lysate using GTX64744 RASGRF1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64744_20180530_ICCIF_18121411_353.jpg

GTX64744 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX64744 RASGRF1 antibody.
Blue : DAPI

Package List Price ($)
$ 319