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RASGRF1 antibody

Anti-RASGRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64744
Anti-RASGRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64744
Anti-RASGRF1 antibody used in Western Blot (WB). GTX64744

Cat. No. GTX64744

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all RASGRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RASGRF1 (NP_001139120.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras protein specific guanine nucleotide releasing factor 1 , CDC25 , CDC25L , GNRP , GRF1 , GRF55 , H-GRF55 , PP13187 , ras-GRF1

Background

The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-RASGRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64744

GTX64744 IHC-P Image

IHC-P analysis of human stomach tissue using GTX64744 RASGRF1 antibody.
Dilution : 1:100

Anti-RASGRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64744

GTX64744 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64744 RASGRF1 antibody.
Blue : DAPI

Anti-RASGRF1 antibody used in Western Blot (WB). GTX64744

GTX64744 WB Image

WB analysis of mouse brain tissue lysate using GTX64744 RASGRF1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399