GeneTex
United States (US)

RASGRP2 antibody

GTX108616_WB_19011507_931.jpg
GTX108616_40093_20150508_IP_19011507_195.jpg
GTX108616_40093_WB_M_liver_19011507_102.jpg
GTX108616_40093_IHC_19011507_440.jpg

Cat No. GTX108616

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Raji , mouse liver

Predict Reactivity

Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RASGRP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ras Guanyl Releasing Protein 2,Caldag-Gefi,Cdc25L,Rasgrp2

Cellular Localization

Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein , Cell junction , synapse , synaptosome (Potential) , Cell projection , ruffle membrane; Peripheral membrane protein (Potential)

Background

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108616_WB_19011507_931.jpg

GTX108616 WB Image

Sample (30 µg of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX108616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108616_40093_20150508_IP_19011507_195.jpg

GTX108616 IP Image

Immunoprecipitation of RASGRP2 protein from Raji whole cell extracts using 5 μg of RASGRP2 antibody (GTX108616).
Western blot analysis was performed using RASGRP2 antibody (GTX108616).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108616_40093_WB_M_liver_19011507_102.jpg

GTX108616 WB Image

Sample (50 µg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108616_40093_IHC_19011507_440.jpg

GTX108616 IHC-P Image

RASGRP2 antibody detects RASGRP2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
RASGRP2 antibody (GTX108616) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169