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RASGRP2 antibody

Orthogonal
Anti-RASGRP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX108616
Anti-RASGRP2 antibody used in Immunoprecipitation (IP). GTX108616
Anti-RASGRP2 antibody used in Western Blot (WB). GTX108616
Anti-RASGRP2 antibody used in Western Blot (WB). GTX108616

Cat. No. GTX108616

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RASGRP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Mouse liver , Raji

Predict Reactivity

Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RASGRP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951644

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAS guanyl releasing protein 2 , CALDAG-GEFI , CDC25L

Cellular Localization

Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein , Cell junction , synapse , synaptosome (Potential) , Cell projection , ruffle membrane; Peripheral membrane protein (Potential)

Background

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RASGRP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX108616

GTX108616 IHC-P Image

RASGRP2 antibody detects RASGRP2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
RASGRP2 antibody (GTX108616) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RASGRP2 antibody used in Immunoprecipitation (IP). GTX108616

GTX108616 IP Image

Immunoprecipitation of RASGRP2 protein from Raji whole cell extracts using 5 μg of RASGRP2 antibody (GTX108616).
Western blot analysis was performed using RASGRP2 antibody (GTX108616).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RASGRP2 antibody used in Western Blot (WB). GTX108616

GTX108616 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RASGRP2 antibody used in Western Blot (WB). GTX108616

GTX108616 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RASGRP2 antibody (GTX108616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

S Miyazaki et al. Biol Pharm Bull 2023; 46 (11) : 1512-1516 RasGRP2 Attenuates Oxygen Deprivation-Induced Autophagy in Vascular Endothelial Cells
Application : WB
Liu Y et al. Front Immunol 2023; 14 : 1100231 RASGRP2 is a potential immune-related biomarker and regulates mitochondrial-dependent apoptosis in lung adenocarcinoma.
Application : IHC-P
Reactivity : Human
Montenont E et al. Blood Adv 2021; 5 (9) : 2362-2374 CRISPR-edited megakaryocytes for rapid screening of platelet gene functions.
Application : WB
Takino JI et al. Sci Rep 2019; 9 (1) : 16717 The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells.
Application : WB
Reactivity : Human
Niemz J et al. Eur J Microbiol Immunol (Bp) 2017; 7 (2) : 112-126 The Guanine-Nucleotide Exchange Factor Caldag Gefi Fine-Tunes Functional Properties of Regulatory T Cells.
Application : WB
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169