Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RASGRP2 antibody (GTX108616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of RASGRP2 protein from Raji whole cell extracts using 5 μg of RASGRP2 antibody (GTX108616).
Western blot analysis was performed using RASGRP2 antibody (GTX108616).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

RASGRP2 antibody detects RASGRP2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
RASGRP2 antibody (GTX108616) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min