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RASGRP3 antibody [N1], N-term

Anti-RASGRP3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104706
Anti-RASGRP3 antibody [N1], N-term used in Western Blot (WB). GTX104706

Cat No. GTX104706

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

A431 , Jurkat , Raji

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RASGRP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RAS guanyl releasing protein 3 , GRP3

Background

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Database

DATA IMAGES
Anti-RASGRP3 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104706

GTX104706 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using RASGRP3 (GTX104706) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RASGRP3 antibody [N1], N-term used in Western Blot (WB). GTX104706

GTX104706 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX104706 diluted at 1:500

REFERENCE
There are currently no references for RASGRP3 antibody [N1], N-term (GTX104706). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RASGRP3 antibody [N1], N-term (GTX104706). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169