GeneTex
United States (US)

RASSF1 antibody [N1C3]

GTX100543_IFA_19011506_599.jpg
GTX100543_WB_19011506_772.jpg

Cat No. GTX100543

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3

Predict Reactivity

Mouse, Rat, Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RASSF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

123F2 antibody, NORE2A antibody, RASSF1A antibody, RDA32 antibody, REH3P21 antibody, RASSF1 antibody, tumor suppressor protein RDA32 antibody, pancreas-specific ras association domain family 1 protein antibody, ras association domain-containing protein 1 antibody, WUGSC:H_LUCA12.5 antibody, cardiac-specific ras association domain family 1 protein antibody, Ras association (RalGDS/AF-6) domain family member 1 antibody

Cellular Localization

Isoform A: Cytoplasm , cytoskeleton , Centrosome , Spindle , Isoform C: Nucleus

Background

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100543_IFA_19011506_599.jpg

GTX100543 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RASSF1(GTX100543) antibody at 1:100 dilution.

GTX100543_WB_19011506_772.jpg

GTX100543 WB Image

Sample(30 ug whole cell lysate)
A:293T
12% SDS PAGE
GTX100543diluted at 1:500

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf