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RASSF1A antibody [3F3]

Anti-RASSF1A antibody [3F3] used in Western Blot (WB). GTX50075
Anti-RASSF1A antibody [3F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50075

Cat No. GTX50075

Host

Mouse

Clonality

Monoclonal

Clone Name

3F3

Isotype

IgG1

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:500 ~ 1:2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human RASSF1A (1-340aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ras Association Domain Family Member 1 , 123F2 , Nore2A , Rassf1A , Rda32 , Reh3P21 , Rassf1

Cellular Localization

Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late

Background

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.

Database

Research Area

DATA IMAGES

Anti-RASSF1A antibody [3F3] used in Western Blot (WB). GTX50075

GTX50075 WB Image

Cell lysates of HEk293(1), A549(2) and HeLa(3) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection

Anti-RASSF1A antibody [3F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50075

GTX50075 ICC/IF Image

A431 cells were labeled with anti-RASSF1A (clone 3F3) mAb, and detection was using a biotinylated secondary antibody and Texas-red conj streptavidin.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf