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RASSF1A antibody [3F3]

Anti-RASSF1A antibody [3F3] used in Western Blot (WB). GTX50075
Anti-RASSF1A antibody [3F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50075

Cat. No. GTX50075

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F3

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all RASSF1 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:500 ~ 1:2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human RASSF1A (1-340aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras association domain family member 1 , 123F2 , NORE2A , RASSF1A , RDA32 , REH3P21

Cellular Localization

Nucleus

Background

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-RASSF1A antibody [3F3] used in Western Blot (WB). GTX50075

GTX50075 WB Image

Cell lysates of HEk293(1), A549(2) and HeLa(3) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection

Anti-RASSF1A antibody [3F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50075

GTX50075 ICC/IF Image

A431 cells were labeled with anti-RASSF1A (clone 3F3) mAb, and detection was using a biotinylated secondary antibody and Texas-red conj streptavidin.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399