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RBBP5 antibody

Anti-RBBP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33461
Anti-RBBP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33461
Anti-RBBP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33461
Anti-RBBP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33461
Anti-RBBP5 antibody used in Western Blot (WB). GTX33461

Cat. No. GTX33461

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RBBP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 319-538 of human RBBP5 (NP_005048.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB binding protein 5, histone lysine methyltransferase complex subunit , RBQ3 , SWD1

Cellular Localization

Nucleus

Background

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-RBBP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33461

GTX33461 ICC/IF Image

ICC/IF analysis of A549 cells using GTX33461 RBBP5 antibody.
Blue : DAPI

Anti-RBBP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33461

GTX33461 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX33461 RBBP5 antibody.
Dilution : 1:100

Anti-RBBP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33461

GTX33461 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX33461 RBBP5 antibody.
Dilution : 1:100

Anti-RBBP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33461

GTX33461 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX33461 RBBP5 antibody.
Dilution : 1:100

Anti-RBBP5 antibody used in Western Blot (WB). GTX33461

GTX33461 WB Image

WB analysis of various sample lysates using GTX33461 RBBP5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399