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RBCK1 antibody

Anti-RBCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115451
Anti-RBCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115451
Anti-RBCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115451
Anti-RBCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115451
Anti-RBCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115451

Cat No. GTX115451

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBCK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885367

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RANBP2-type and C3HC4-type zinc finger containing 1 , C20orf18 , HOIL-1 , HOIL1 , PBMEI , PGBM1 , RBCK2 , RNF54 , UBCE7IP3 , XAP3 , XAP4 , ZRANB4

Background

The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq]

Database

DATA IMAGES

Anti-RBCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115451

GTX115451 IHC-P Image

RBCK1 antibody detects RBCK1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
RBCK1 antibody (GTX115451) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RBCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115451

GTX115451 ICC/IF Image

RBCK1 antibody detects RBCK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RBCK1 protein stained by RBCK1 antibody (GTX115451) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-RBCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115451

GTX115451 IHC-P Image

RBCK1 antibody detects RBCK1 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
RBCK1 antibody (GTX115451) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RBCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115451

GTX115451 IHC-P Image

RBCK1 antibody detects RBCK1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RBCK1 antibody (GTX115451) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RBCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115451

GTX115451 IHC-P Image

RBCK1 antibody detects RBCK1 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
RBCK1 antibody (GTX115451) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169