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RBFA antibody

Orthogonal
Anti-RBFA antibody used in Western Blot (WB). GTX131133
Anti-RBFA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131133

Cat. No. GTX131133

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RBFA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBFA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886429

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosome binding factor A , C18orf22 , HsT169

Cellular Localization

Mitochondrion

Database

DATA IMAGES

Anti-RBFA antibody used in Western Blot (WB). GTX131133

GTX131133 WB Image

Orthogonal

HeLa whole cell and cytoplasm extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBFA antibody (GTX131133) diluted at 1:1000.

Anti-RBFA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131133

GTX131133 ICC/IF Image

RBFA antibody detects RBFA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBFA protein stained by RBFA antibody (GTX131133) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169