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RBM15 antibody [N1N3]

Orthogonal
Anti-RBM15 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109899
Anti-RBM15 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109899
Anti-RBM15 antibody [N1N3] used in Western Blot (WB). GTX109899

Cat No. GTX109899

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all RBM15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBM15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951657

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA binding motif protein 15 , OTT , OTT1 , SPEN

Cellular Localization

Nucleus

Background

Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RBM15 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109899

GTX109899 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RBM15(GTX109899) antibody at 1:200 dilution.

Anti-RBM15 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109899

GTX109899 IHC-P Image

RBM15 antibody [N1N3] detects RBM15 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RBM15 stained by RBM15 antibody [N1N3] (GTX109899) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBM15 antibody [N1N3] used in Western Blot (WB). GTX109899

GTX109899 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RBM15 antibody [N1N3] (GTX109899) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169