APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , Neuro2A , GL261 , C8D30
Predict Reactivity
Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RBM4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11175358
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
RNA binding motif protein 4 , LARK , RBM4A , ZCCHC21 , ZCRB3A
Cellular Localization
Nucleus , nucleolus , Cytoplasm
Background
May play a role in alternative splice site selection during pre-mRNA processing.
Database
Research Area
DATA IMAGES
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GTX121565 WB Image
Wild-type (WT) and RBM4 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBM4 antibody (GTX121565) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX121565 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RBM4 antibody (GTX121565) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX121565 WB Image
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX121565 diluted at 1:5000
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REFERENCE
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REVIEW
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