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RBM4 antibody

KO/KD
Anti-RBM4 antibody used in Western Blot (WB). GTX121565
Anti-RBM4 antibody used in Western Blot (WB). GTX121565
Anti-RBM4 antibody used in Western Blot (WB). GTX121565

Cat No. GTX121565

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all RBM4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLa , Neuro2A , GL261 , C8D30

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBM4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175358

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA binding motif protein 4 , LARK , RBM4A , ZCCHC21 , ZCRB3A

Cellular Localization

Nucleus , nucleolus , Cytoplasm

Background

May play a role in alternative splice site selection during pre-mRNA processing.

Database

Research Area

DATA IMAGES

Anti-RBM4 antibody used in Western Blot (WB). GTX121565

GTX121565 WB Image

KO/KD

Wild-type (WT) and RBM4 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBM4 antibody (GTX121565) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RBM4 antibody used in Western Blot (WB). GTX121565

GTX121565 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with RBM4 antibody (GTX121565) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBM4 antibody used in Western Blot (WB). GTX121565

GTX121565 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX121565 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169