APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
K562 , THP-1
Predict Reactivity
Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RBM5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886088
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RNA binding motif protein 5 , G15 , H37 , LUCA-15 , LUCA15 , RMB5
Cellular Localization
Nucleus
Background
Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.
Database
Research Area
DATA IMAGES
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GTX129783 IP Image
Immunoprecipitation of RBM5 protein from 293T whole cell extracts using 5 μg of RBM5 antibody (GTX129783).
Western blot analysis was performed using RBM5 antibody (GTX129783).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX129783 WB Image
RBM5 antibody detects RBM5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5 % SDS-PAGE, and blotted with RBM5 antibody (GTX129783) diluted by 1:1000
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REFERENCE
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REVIEW
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