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RBM8A antibody

Anti-RBM8A antibody used in IHC (Paraffin sections) (IHC-P). GTX131387
Anti-RBM8A antibody used in IHC (Paraffin sections) (IHC-P). GTX131387
Anti-RBM8A antibody used in Western Blot (WB). GTX131387
Anti-RBM8A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131387

Cat. No. GTX131387

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Zebrafish, Bovine, Japanese Medaka, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBM8A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886473

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA binding motif protein 8A , BOV-1A , BOV-1B , BOV-1C , C1DELq21.1 , DEL1q21.1 , MDS014 , RBM8 , RBM8B , TAR , Y14 , ZNRP , ZRNP1

Cellular Localization

Nucleus , Nucleus speckle , Cytoplasm

Background

This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RBM8A antibody used in IHC (Paraffin sections) (IHC-P). GTX131387

GTX131387 IHC-P Image

RBM8A antibody detects RBM8A protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RBM8A antibody (GTX131387) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBM8A antibody used in IHC (Paraffin sections) (IHC-P). GTX131387

GTX131387 IHC-P Image

RBM8A antibody detects RBM8A protein at cytoplasm and nucleus in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
RBM8A antibody (GTX131387) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBM8A antibody used in Western Blot (WB). GTX131387

GTX131387 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RBM8A antibody (GTX131387) diluted at 1:1000.

Anti-RBM8A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131387

GTX131387 ICC/IF Image

RBM8A antibody detects RBM8A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBM8A protein stained by RBM8A antibody (GTX131387) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169