GeneTex
United States (US)

RBP1 antibody

GTX108016_39735_IFA_19011507_379.jpg
GTX108016_WB_19011507_657.jpg
GTX108016_39735_WB_M_19011507_920.jpg
GTX108016_39735_IHC_19011507_259.jpg

Cat No. GTX108016

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

H1299 , NIH-3T3 , C2C12

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human RBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Retinol Binding Protein 1,Crabp-I,Crbp,Crbp1,Crbpi,Rbpc,Rbp1

Cellular Localization

Cytoplasm

Background

This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108016_39735_IFA_19011507_379.jpg

GTX108016 ICC/IF Image

Immunofluorescence analysis of methanol-fixed H1299, using CRBP I(GTX108016) antibody at 1:500 dilution.

GTX108016_WB_19011507_657.jpg

GTX108016 WB Image

Sample (30 µg of whole cell lysate)
A: H1299
15% SDS PAGE
GTX108016 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108016_39735_WB_M_19011507_920.jpg

GTX108016 WB Image

CRBP I antibody detects RBP1 protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extract
12% SDS-PAGE
CRBP I antibody (GTX108016) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108016_39735_IHC_19011507_259.jpg

GTX108016 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using CRBP I(GTX108016) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169