GeneTex
United States (US)

RBP3 antibody [C2C3], C-term

GTX114714_40219_20160118_IHC-P_M_18121410_768.jpg
GTX114714_40219_20151030_IFA_18121410_281.jpg

Cat No. GTX114714

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

135 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RBP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Retinol Binding Protein 3,D10S64,D10S65,D10S66,Irbp,Rbpi,Rp66,Rbp3

Cellular Localization

Secreted , extracellular space , extracellular matrix , interphotoreceptor matrix

Background

Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114714_40219_20160118_IHC-P_M_18121410_768.jpg

GTX114714 IHC-P Image

RBP3 antibody [C2C3], C-term detects RBP3 protein at cytoplasm in mouse eye by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
RBP3 antibody [C2C3], C-term (GTX114714) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114714_40219_20151030_IFA_18121410_281.jpg

GTX114714 ICC/IF Image

RBP3 antibody [C2C3], C-term detects RBP3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RBP3 protein stained by RBP3 antibody [C2C3], C-term (GTX114714) diluted at 1:400.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169