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RBP4 antibody

Anti-RBP4 antibody used in Western Blot (WB). GTX110632
Anti-RBP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110632
Anti-RBP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX110632

Cat. No. GTX110632

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RBP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Cat, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951665

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

retinol binding protein 4 , MCOPCB10 , RDCCAS

Cellular Localization

Secreted

Background

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RBP4 antibody used in Western Blot (WB). GTX110632

GTX110632 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
12% SDS PAGE
GTX110632 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110632

GTX110632 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RBP4(GTX110632) antibody at 1:200 dilution.

Anti-RBP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX110632

GTX110632 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OECM1 xenograft, using RBP4(GTX110632) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169