APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human RBPJK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951667
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
recombination signal binding protein for immunoglobulin kappa J region , AOS3 , CBF1 , IGKJRB , IGKJRB1 , KBF2 , RBP-J , RBPJK , RBPSUH , SUH , csl
Cellular Localization
Nucleus
Background
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate. determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some Notch protein, it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.
Database
Research Area
DATA IMAGES
![Anti-RBPJK antibody [C1C3] used in Western Blot (WB). GTX112358](/templates/default/js/lightbox/images/loading.gif "Anti-RBPJK antibody [C1C3] used in Western Blot (WB). GTX112358")
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GTX112358 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBPJK antibody [C1C3] (GTX112358) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody,
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GTX112358 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RBP-Jkappa(GTX112358) antibody at 1:100 dilution.
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REFERENCE
There are currently no references for RBPJK antibody [C1C3] (GTX112358). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RBPJK antibody [C1C3] (GTX112358). Be the first to share your experience with this product.
![Anti-RBPJK antibody [C1C3] used in Western Blot (WB). GTX112358](/upload/website/prouct_img/small/GTX112358/GTX112358_44216_20210226_WB_w_23060500_245.webp "Anti-RBPJK antibody [C1C3] used in Western Blot (WB). GTX112358")
![Anti-RBPJK antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112358](/upload/website/prouct_img/small/GTX112358/GTX112358_IFA_w_23060500_639.webp "Anti-RBPJK antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112358")
![Anti-RBPJK antibody [C1C3] used in Western Blot (WB). GTX112358](/upload/website/prouct_img/normal/GTX112358/GTX112358_44216_20210226_WB_w_23060500_245.webp "Anti-RBPJK antibody [C1C3] used in Western Blot (WB). GTX112358")
![Anti-RBPJK antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112358](/upload/website/prouct_img/normal/GTX112358/GTX112358_IFA_w_23060500_639.webp "Anti-RBPJK antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112358")