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RBPJK antibody [C1C3]

Anti-RBPJK antibody [C1C3] used in Western Blot (WB). GTX112358
Anti-RBPJK antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112358

Cat. No. GTX112358

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBPJK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951667

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

recombination signal binding protein for immunoglobulin kappa J region , AOS3 , CBF1 , IGKJRB , IGKJRB1 , KBF2 , RBP-J , RBPJK , RBPSUH , SUH , csl

Cellular Localization

Nucleus

Background

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate. determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some Notch protein, it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence.

Database

Research Area

DATA IMAGES

Anti-RBPJK antibody [C1C3] used in Western Blot (WB). GTX112358

GTX112358 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBPJK antibody [C1C3] (GTX112358) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody,

Anti-RBPJK antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112358

GTX112358 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RBP-Jkappa(GTX112358) antibody at 1:100 dilution.

REFERENCE

There are currently no references for RBPJK antibody [C1C3] (GTX112358). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RBPJK antibody [C1C3] (GTX112358). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169