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RBPMS antibody [HL1105]

Recombinant antibody KO/KD
Anti-RBPMS antibody [HL1105] used in Western Blot (WB). GTX636320
Anti-RBPMS antibody [HL1105] used in IHC (Paraffin sections) (IHC-P). GTX636320
Anti-RBPMS antibody [HL1105] used in IHC (Paraffin sections) (IHC-P). GTX636320
Anti-RBPMS antibody [HL1105] used in IHC (Paraffin sections) (IHC-P). GTX636320
Anti-RBPMS antibody [HL1105] used in IHC (Paraffin sections) (IHC-P). GTX636320
Anti-RBPMS antibody [HL1105] used in Western Blot (WB). GTX636320
Anti-RBPMS antibody [HL1105] used in Western Blot (WB). GTX636320

Cat. No. GTX636320

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1105

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RBPMS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBPMS. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909965

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding protein, mRNA processing factor , HERMES

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RNA recognition motif family of RNA-binding proteins. The RNA recognition motif is between 80-100 amino acids in length and family members contain one to four copies of the motif. The RNA recognition motif consists of two short stretches of conserved sequence, as well as a few highly conserved hydrophobic residues. The encoded protein has a single, putative RNA recognition motif in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-RBPMS antibody [HL1105] used in Western Blot (WB). GTX636320

GTX636320 WB Image

KO/KD

Non-transfected (–) and transfected (+) HCT116 whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody [HL1105] (GTX636320) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RBPMS antibody [HL1105] used in IHC (Paraffin sections) (IHC-P). GTX636320

GTX636320 IHC-P Image

RBPMS antibody [HL1105] detects RBPMS protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
RBPMS stained by RBPMS antibody [HL1105] (GTX636320) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody [HL1105] used in IHC (Paraffin sections) (IHC-P). GTX636320

GTX636320 IHC-P Image

RBPMS antibody [HL1105] detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: RBPMS stained by RBPMS antibody [HL1105] (GTX636320) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody [HL1105] used in IHC (Paraffin sections) (IHC-P). GTX636320

GTX636320 IHC-P Image

RBPMS antibody [HL1105] detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: RBPMS stained by RBPMS antibody [HL1105] (GTX636320) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody [HL1105] used in IHC (Paraffin sections) (IHC-P). GTX636320

GTX636320 IHC-P Image

RBPMS antibody [HL1105] detects RBPMS protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RBPMS stained by RBPMS antibody [HL1105] (GTX636320) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody [HL1105] used in Western Blot (WB). GTX636320

GTX636320 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody [HL1105] (GTX636320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody [HL1105] used in Western Blot (WB). GTX636320

GTX636320 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody [HL1105] (GTX636320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations