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RBPMS antibody

Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619

Cat. No. GTX118619

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBPMS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720427

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding protein, mRNA processing factor , HERMES

Background

This gene encodes a member of the RRM family of RNA-binding proteins. The RRM domain is between 80-100 amino acids in length and family members contain one to four copies of the domain. The RRM domain consists of two short stretches of conserved sequence called RNP1 and RNP2, as well as a few highly conserved hydrophobic residues. The protein encoded by this gene has a single, putative RRM domain in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Non-transfected (–) and transfected (+) HCT-116 whole cell extract were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118619

GTX118619 ICC/IF Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118619

GTX118619 ICC/IF Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: RBPMS protein stained by RBPMS antibody (GTX118619) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

The data was published in the journal Cell Death Discov in 2019. PMID: 31285855

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

The data was published in the journal PLoS One in 2018.PMID: 29401485

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169