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RBPMS antibody

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619
Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619
Anti-RBPMS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118619
Anti-RBPMS antibody used in Western Blot (WB). GTX118619

Cat No. GTX118619

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A549 , mouse heart

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBPMS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720427

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA binding protein, mRNA processing factor , HERMES

Background

This gene encodes a member of the RRM family of RNA-binding proteins. The RRM domain is between 80-100 amino acids in length and family members contain one to four copies of the domain. The RRM domain consists of two short stretches of conserved sequence called RNP1 and RNP2, as well as a few highly conserved hydrophobic residues. The protein encoded by this gene has a single, putative RRM domain in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
RBPMS antibody (GTX118619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: RBPMS protein stained by RBPMS antibody (GTX118619) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
RBPMS stained by RBPMS antibody (GTX118619) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBPMS antibody used in IHC (Paraffin sections) (IHC-P). GTX118619

GTX118619 IHC-P Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
RBPMS antibody (GTX118619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RBPMS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118619

GTX118619 ICC/IF Image

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBPMS protein stained by RBPMS antibody (GTX118619) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RBPMS antibody used in Western Blot (WB). GTX118619

GTX118619 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (GTX118619) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

RBPMS antibody Cat No. GTX118619

Rating ( Average 5 based on 18 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 10 users reviews)
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 6 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2018.PMID: 29401485
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2020.PMID: 32350384
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2020.PMID: 32350384
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2020.PMID: 32350384
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Mol Neurodegener in 2015.PMID: 26306916
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Mol Neurodegener in 2016.PMID: 27008854
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Cell Death Discov in 2019.PMID: 31285855
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Invest Ophthalmol Vis Sci in 2019.PMID: 30601926
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Invest Ophthalmol Vis Sci in 2019.PMID: 30601926
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Invest Ophthalmol Vis Sci in 2019.PMID: 30601926
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Invest Ophthalmol Vis Sci in 2019.PMID: 30601926
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Invest Ophthalmol Vis Sci in 2019.PMID: 30601926
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Invest Ophthalmol Vis Sci in 2019.PMID: 30601926
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2019.PMID: 30930743
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2019.PMID: 30930743
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2019.PMID: 30930743
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2019.PMID: 30930743
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2019.PMID: 30930743
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf