Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RBX1 antibody (GTX110507) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX110507 diluted at 1:1000