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RBX1 antibody

Anti-RBX1 antibody used in Western Blot (WB). GTX110507
Anti-RBX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110507

Cat No. GTX110507

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RBX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ring-Box 1,Ba554C12.1,Rnf75,Roc1,Rbx1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-RBX1 antibody used in Western Blot (WB). GTX110507

GTX110507 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX110507 diluted at 1:1000

Anti-RBX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110507

GTX110507 ICC/IF Image

RBX1 antibody detects RBX1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBX1 protein stained by RBX1 antibody (GTX110507) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169