APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RBX1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BA554C12.1 antibody, FLJ60363 antibody, MGC13357 antibody, MGC1481 antibody, RNF75 antibody, ROC1 antibody, RBX1 antibody, RING-box protein 1 antibody, regulator of cullins 1 antibody, ZYP protein antibody, E3 ubiquitin-protein ligase RBX1 antibody, RING finger protein 75 antibody, RING box protein 1 antibody, "ring-box 1, E3 ubiquitin protein ligase antibody"
Cellular Localization
Cytoplasm , Nucleus
Background
This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX110507 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX110507 diluted at 1:1000
|
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GTX110507 ICC/IF Image
RBX1 antibody detects RBX1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBX1 protein stained by RBX1 antibody (GTX110507) diluted at 1:500.
Blue: Hoechst 33343 staining.
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