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RBX1 antibody

KO/KD
Anti-RBX1 antibody used in Western Blot (WB). GTX110507
Anti-RBX1 antibody used in Western Blot (WB). GTX110507

Cat No. GTX110507

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all RBX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RBX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037896

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ring-box 1 , BA554C12.1 , RNF75 , ROC1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RBX1 antibody used in Western Blot (WB). GTX110507

GTX110507 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RBX1 antibody (GTX110507) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RBX1 antibody used in Western Blot (WB). GTX110507

GTX110507 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX110507 diluted at 1:1000

REFERENCE

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REVIEW

RBX1 antibody Cat No. GTX110507

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-RBX1 antibody used in Western Blot (WB). GTX110507
Date : Anonymous submitted on 06-Nov-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : hTERT-RPE1 cells
Amount used : 10 μg
Blocking : 5% dry fat milk in PBS/0.05% Tween-20, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169