GeneTex
United States (US)

RBX1 antibody

GTX110507_39988_WB_M_Brain_19011507_380.jpg
GTX110507_39988_IFA_19011507_710.jpg

Cat No. GTX110507

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RBX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BA554C12.1 antibody, FLJ60363 antibody, MGC13357 antibody, MGC1481 antibody, RNF75 antibody, ROC1 antibody, RBX1 antibody, RING-box protein 1 antibody, regulator of cullins 1 antibody, ZYP protein antibody, E3 ubiquitin-protein ligase RBX1 antibody, RING finger protein 75 antibody, RING box protein 1 antibody, "ring-box 1, E3 ubiquitin protein ligase antibody"

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX110507_39988_WB_M_Brain_19011507_380.jpg

GTX110507 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX110507 diluted at 1:1000

GTX110507_39988_IFA_19011507_710.jpg

GTX110507 ICC/IF Image

RBX1 antibody detects RBX1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBX1 protein stained by RBX1 antibody (GTX110507) diluted at 1:500.
Blue: Hoechst 33343 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169